Journal: Phytotherapy research : PTR

Article Title: Pterostilbene Exhibits Broad-Spectrum Antiviral Activity by Targeting the Enterovirus Capsid, Inactivating Viral Particles, Blocking Viral Binding, and Protecting Mice From Lethal EV-A71 Challenge.

doi: 10.1002/ptr.8496

Figure Lengend Snippet: FIGURE 8 | Oral administration of pterostilbene protects against EV-A71 infection. (a) Schematic diagram of in vivo experiment. hSCARB2 mice (21-d-old) were intragrastrically (i.g.) infected with EV-A71, and then inoculated (i.g.) once-daily with 20 and 50 mg/kg Pte for 1 h after Day 0–6, consecutively for a total of 7 day. (b) Representative photographs of healthy mice in the EV-A71 + Pte (50 mg/kg) group. In contrast, limb paralysis was observed among mice in the EV-A71 alone group. (c) Body weight, (d) survival rate, and (e) clinical scores were recorded daily after infection for 14 day. Clinical scores were graded as: 0, healthy; 1, hair loss, wasting, or ruffled hair; 2, limb weakness; 3, paralysis in only one limb; 4, paralysis in 2–4 limbs; 5, death. N = 6–7. Data are expressed as the mean ± SEM. **: p < 0.01, ***: p < 0.001 versus the virus control group.

Article Snippet: The mixtures were then incubated with the prewashed protein- conjugated beads for 2 h. Next, the beads were washed thrice and mixed with sample buffer at 90°C for 10 min. Precipitated viruses and proteins were quantified via western blotting using anti- EV- A71 VP1, anti- EV- D68 VP1, mouse anti- hPSGL- 1 (sc- 13535; 1:3000; Santa Cruz, Dallas, TX, USA), mouse anti- hSCARB2 (sc- 55571; 1:2500; Santa Cruz), and mouse anti- hICAM- 5 (1 μg/mL; MAB- 1950; R&D Systems) antibodies (Hsieh et al. 2022).

Techniques: Infection, In Vivo, Virus, Control